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Next Generation Sequencing & Data Analysis

Introduction

Next Generation Sequencing and Data Analysis is Organized by REVA University, School of Physical, Chemical and Biological Sciences, Department of Biotechnology and Scientific Bio-Minds, Bangalore, 09-14, April, 2018. The cost of next generation sequencing (NGS) has fallen rapidly, allowing NGS to be routinely applied within research or clinical genetics laboratories. Whole Exome Sequencing (WES) utilizes NGS to sequence all the protein coding regions of the genome in a single assay. Performing WES can be more cost effective than performing multiple, smaller sequencing experiments, each with a panel of genes. However, adoption of NGS introduces new bioinformatics challenges to laboratories, requiring a new skill set to enable data to be processed, analyzed and interpreted correctly.

Course Objectives

This workshop will introduce two modules:

  • Sequencing (Sample selection, DNA Isolation, Library preparation, sequencing)
  • Sequencing Data Analysis with the UNIX and LINUX command line, the platform on which many bioinformatics tools are designed to run on, before outlining and exploring the steps of a bioinformatics pipeline.

Course Syllabus

Module: 1

  • Understanding the concepts of NGS
  • Experimental design of next generation sequencing projects
  • Sample collection, storage, transportation
  • Genomic DNA isolation
  • Genomic DNA Sample size estimation
  • NGS sample library Preparation
  • Sequencing of genomic DNA

Module 2 (Bioinformatics and Big Data Analytics)

  • Understanding and learning basic commends on UNIX and LINUX
  • NGS Sequence data file formates, file conversions
  • Quality analysis, removal of adapters, sequence trimming
  • Sequence annotation and mapping
  • Differential gene expression analysis
  • Comparative genomics
  • Data submissions & graphical representations
  • NGS data submission to NCBI
Download Brochure Registration Form

Registration Fees

Last date for Registration: 05 April, 2018

Module: 1

Students: 3500/-
Faculty Members: 5000/-
Industrial People: 7000/-

Module: 2

Students: 3500/-
Faculty Members: 5000/-
Industrial People: 7000/-

Both

Students: 7000/-
Faculty Members: 10000/-
Industrial People: 14000/-

Registration Procedure

Call/ SMS / Whatsapp to +91-9844158444 Or Email with Name, Phone number, Email address, University/College/Institution, Address, Field of study, References to scientificbiominds@gmail.com web link www.scientificbiominds.in

Facilities Available

  • Participants are encouraged to bring their own laptops with UBUNTU system
  • Wifi facility is provided
  • Participants need accommadation please contact Dr.C.N.Prashanth +91-9844158444
  • Study materials, Workshop related softwares will be provided

Please Download the application form, fill and send to our official address.